Osmiophilic reagents in electronmicroscopic histocytochemistry by Jacob S. Hanker

Cover of: Osmiophilic reagents in electronmicroscopic histocytochemistry | Jacob S. Hanker

Published by Fischer in Stuttgart, New York .

Written in English

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Subjects:

  • Stains and staining (Microscopy),
  • Electron microscopy -- Technique.,
  • Histochemistry -- Technique.,
  • Cytochemistry -- Technique.,
  • Chemical tests and reagents.

Edition Notes

Book details

StatementJacob S. Hanker.
SeriesProgress in histochemistry and cytochemistry ;, v. 12, no. 1
Classifications
LC ClassificationsQH237 .H35
The Physical Object
Pagination85 p. :
Number of Pages85
ID Numbers
Open LibraryOL3906973M
ISBN 100895740796
LC Control Number81469727
OCLC/WorldCa8050624

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Osmiophilic reagents in electronmicroscopic histocytochemistry. Hanker JS. Direct histocytochemical staining methods on undisrupted tissues, stabilized bychemical fixation, potentially offer perhaps the most reliable approach to thestudy of the enzymes of the cell with relation to its ultrastructure.

The atomswhich, for the most part, comprise the biomacromolecules and enzymes of cells andtissues contribute little to their inherent electron opacity or ability toscatter electrons Cited by: COVID campus closures: see options for getting or retaining Remote Access to subscribed contentCited by: Additional Physical Format: Online version: Hanker, Jacob S.

Osmiophilic reagents in electronmicroscopic histocytochemistry. Stuttgart ; New York: Fischer, Osmiophilic reagents in electron microscopic histochemistry progress in histochemistry and cytochemistry, Vol.

1, no. 1 Jacob S. Hanker Stutigart, New York, Gustav Fischer Verlag,DM. (9) in our reagents for the cytochemi-cal demonstration of enzymes and func-tional groups. A preliminary survey of reagents containing osmiophilic groups Osmiophilic reagents in electronmicroscopic histocytochemistry book that thiols reduced OsO more iodine-carbon bond in the kv elec- readily than alkenes, alkynes, aryla-Fig.

Guinea pig spleen (polymorphonuclear heterophil) fixed in glutaraldehyde,Cited by: Osmiophilic reagents in electronmicroscopic histocytochemistry Jacob S. Hanker (Progress in histochemistry and cytochemistry, v.

12, no. 1) Fischer, N.Y. The alkali metals (Li, Na, K etc.) and the alkaline earth metals (Mg and Ca, together with Zn) are good reducing agents, the former being stronger than the latter.

These same metals reduce the carbon-halogen bonds of alkyl halides. The halogen is converted to a halide anion, and the carbon bonds to the metal which has characteristics similar to a carbanion (R:).

Electrophilic addition Start Reagents HCl HBr HI H 2 O/H + H Osmiophilic reagents in electronmicroscopic histocytochemistry book first H ends up on less substituted end. Nucleophile attacks more substituted end Markovnikov product 1 Halogen atom added Reaction intermediate has 3 possible structures NEGLIGIBLE Halonium ion.

Abstract. The idea that neuroactive substances may coexist in neurones is not new and indeed probably goes back as far as the original hypothesis of Burn and Rand () concerning the presence of acetylcholine (ACh) along with noradrenaline (NA) in certain postganglionic sympathetic nerves.

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THE detail seen in a highly magnified electron micrograph is to-day limited more by a lack of contrast in the image than by any lack of resolution in the microscope, which is now usually capable. A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections Daniel D.

Dunning, John G. McHaffie, and Barry E. Stein Journal of Histochemistry & Cytochemistry 6, Graham, R. C., Jr & Karnovsky, M. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Bussolati G () Histochemical demonstration of sulfate groups by means of diaminobenzidine. Histochem J – Google Scholar. Hanker, J.S. et al (). Osmiophilic reagents: new cytochemical principles for light and electron microscopy.

Science New York, Derenzini, M. et al (). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glycoconjugates at the molecular level in-situ.

Histochem. Cytochem., Europe PMC is an archive of life sciences journal literature. The review presents applications of 3,3'-diaminobenzidine (DAB) to histochemistry and cytochemistry, including demonstration of oxidative enzymes and hemoproteins, tracer experiments, immunocytochemistry and methods based on non-enzymic DAB oxidation.

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Histological grade chemicals are commonly used as laboratory solvents. Osmiophilic reagents in electronmicroscopic histocytochemistry. Hanker JS. Prog Histochem Cytochem, 12(1), 01 Jan Cited by: 12 articles | PMID: Review.

Distribution and determination of cholinesterases in mammals. Holmstedt B. Bull World. organolithium reagents are most commonly used as very strong bases and in reactions with carbonyl compounds R-X 2R-M R-H + M-OH (0)M HO.

2 Preparation of Organomagnesium Compounds: Grignard Reagents R-X R-MgX (Grignard reagent) Mg(0) THF R-X can be an alkyl, vinyl, or aryl halide (chloride, bromide. Mouse LIMR3/CDf is a negative regulator of the antimicrobial activity of neutrophils J.

Osmiophilic reagents in electronmicroscopic histocytochemistry. is a negative regulator of the. A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria. Classical biochemical tests are often used to identify microorganisms; the results are seen by color change.

In most cases, detection is based on the reaction of an enzyme with a certain substrate. Also, methods to detect certain metabolites by chemical.

Exercise stimulates immune responses, but the appropriate “doses” for such achievements are unsettled. Conversely, in metabolic tissues, exercise improves the heat shock (HS) response, a universal cytoprotective response to proteostasis challenges that are centred on the expression of the kDa family of intracellular heat shock proteins (iHSP70), which are anti-inflammatory.

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Excellent for the molecular biology industry, our reagents ensure accuracy and top of the. Facilitated light microscopic cytochemical diagnosis of acute myelogenous leukemia Osmiophilic Reagents in Electronmicroscopic Histocytochemistry. Since the introduction of osmiophilic.

Europe PMC is an archive of life sciences journal literature. Galactosylated BSA (galBSA) and its conjugate to horseradish peroxidase (galBSA-HRP) enter the galactose-specific pathway of hepatocytes. 10 min after intravenous injection, structures containing either ligand sediment mostly betw and 3 X 10(6) g X min (LP fraction) and have an equilibrium density of g/ml in.

Myzozoans (which include dinoflagellates, chromerids and apicomplexans) display notable divergence from their ciliate sister group, including a reduced mitochondrial genome and divergent metabolic.

Osmiophilic polymer generation: catalysis by transition metal compounds in ultrastructural cytochemistry. Hanker JS, Anderson WA, Bloom FE. A transition metal compound that is bound in tissues by any appropriate cytochemical reaction may catalyze the generation of an insoluble osmiophilic polymer from organic monomers such as 3,3'-diaminobenzidine.

We’d like to understand how you use our websites in order to improve them. NONDROPLET ULTRASTRUCTURAL DEMONSTRATION OF CYTOCHROME OXIDASE ACTIVITY WITH A POLYMERIZING OSMIOPHILIC REAGENT, DIAMINOBENZIDINE (DAB) In electron microscopic preparations the excess reaction product is found to overflow into the intracristate spaces and into the outer compartment between inner and outer limiting mitochondrial membranes.

Localization of Lactic Dehydrogenase Isozymes in Lysosomal Fraction of the Neutrophil of Normal Human Blood Skip to main content Thank you for visiting Jacob S.

Hanker's research works with 4, citations and 1, reads, including: Increased Candida Demonstrated in Subgingival Plaques of Aids Patients by Microwave-Accelerated Silver Staining. Cytochemical marker of differentiation in acute leukemia.

Osmiophilic Reagents in Electronmicroscopic Histocytochemistry Perhaps the most widely employed osmiophilic reagent in. 1, * Secondary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation.

DOT Scientific Inc., Lippincott Blvd, () () (Toll free number). The periodontal ligament receives a rich sensory nerve supply and contains many nociceptors and mechanoreceptors. Although its various kinds of mechanoreceptors have been reported in the past, only recently have studies revealed that the Ruffini endings-categorized as low-threshold, slowly adapting, type II mechanoreceptors-are the primary mechanoreceptors in the periodontal ligament.

HISTOCHEMISTRY OF CREATINE PHOSPHOKINASE Histochemical fixation Fresh 2 % formaldehyde was prepared by heat depolymerization of paraformalde hyde [45]. The fixative was buffered with m sodium cacodylate (pH ) containing 6% glucose and 6% dextran, and 10 mm of cysteine was added to reduce loss of CPK activity [42].

High resolution cytochemical staining of polysaccharides in the cells of the bean fuscous blight bacteria Xanthomonas fuscans (Burkholder) Bur. revealed extensive deposits of a periodic acid-oxidizable compound in the cytoplasm, which accumulated at the onset of polysaccharide excretion and occupied a substantial part of the cell volume.

In the course of subsequent culture growth the. Acid Halogenation Reagents. By: Matthias Junkers, ChemFiles, 3.

The generation of an acid chloride is an obvious way to activate the carboxy group for amide bond formation. However, practical application of acid chlorides in peptide synthesis is restricted, because they are prone to side reactions and racemization.

Acta histochem, Ed. 56, S. () Laboratory of Histology, Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan The reactions of rabbit gall bladder epithelium to electron micro­SCOpIC stainings for carbohydrates with 1,2-glycol groupings By KAZUYORI YAMADA and MrTSUKANE HOSHINO With 12 figures (Received Octo ) Summary In the rabbit gall .Group specific reagents are enzyme inhibitors that can covalently bond to a particular amino acid residue on the enzyme and irreversibly modify is less specific compared with affinity label and suicide inhibitor.

It reacts with side chain of amino acid.ISBN: OCLC Number: Description: xiv, pages: illustrations ; 25 cm: Contents: Introduction and overview of electron microscopic immunocytochemical procedures / J.V. Priestley --Electron microscopic immunocytochemistry in histopathology / C.H.S. Cameron and P.G.

Toner --Post-embedding electron microscopic immunocytochemistry / A. Merighi --Pre-embedding.

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